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Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
《医学前沿(英文)》 页码 939-956 doi: 10.1007/s11684-023-0987-9
关键词: mononuclear diploid cardiomyocytes cell–cell communication cardiac fibroblast single-cell RNA sequencing cardiac regeneration
Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission
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《医学前沿(英文)》 2017年 第11卷 第4期 页码 548-553 doi: 10.1007/s11684-017-0597-5
This study determined the effect of hepatitis B virus (HBV) replication in peripheral blood mononuclear cell (PBMC) from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg-positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA (rcDNA), and covalently closed circular DNA (cccDNA) were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi-square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% (12/150) in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cccDNA in the HBsAg-positive mothers were 36.67% (55/150) and 10% (15/150), respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission (OR= 6.003, 95% CI: 1.249–28.855). Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA (OR= 3.896, 95% CI: 1.929–7.876) and PBMC HBV cccDNA (OR= 3.74, 95% CI: 1.186–11.793) in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor of PBMC HBV cccDNA (OR= 0.312, 95% CI: 0.102–0.954) during pregnancy. The positivity of PBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers (c2=5.087, P= 0.024). This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.
关键词: PBMC HBV cccDNA HBV rcDNA HBV intrauterine transmission
刘少军,曹运长,何晓晓,李建中,刘筠
《中国工程科学》 2001年 第3卷 第12期 页码 33-41
对雌雄两性能育并形成群体的异源四倍体鲫鲤的染色体数目和组型,DNA含量,红细胞大小,生殖腺和配子,胚胎发育,形成机理,外形等方面进行了较全面描述。四倍体鲫鲤经过九代(F3-F11)的四倍体性的繁殖,已形成了一个数目庞大的遗传性状稳定的群体。该四倍体群体在染色体数目、生殖、外形特征等方面都与它们的原始父母本-二倍体湘江野鲤和红鲫有本质的差别。在遗传特性、稳定传代、生育隔离等方面,异源四倍体鲫鲤群体为形成一个新的四倍体新种奠定了基础。新的四倍体鱼群体的形成对脊椎动物的进化理论和它们在生产上的应用都具有重要的意义。
外周血免疫特征预测肝内胆管癌患者化疗敏感性 Article
吴彤, 杨应成, 郑博, 施学兵, 李炜, 马文聪, 王珊, 李祉萱, 朱妍静, 吴建民, 汪凯婷, 赵燕, 伍睿, 隋成军, 莘似韵, 吴璇, 陈磊, 袁振刚, 王红阳
《工程(英文)》 2021年 第7卷 第10期 页码 1381-1393 doi: 10.1016/j.eng.2021.01.014
肝内胆管癌(ICC)是第二常见肝癌。化疗仍然是晚期ICC患者的主要治疗方法,但是化疗敏感性因人而异。在本研究中,我们使用飞行时间质谱仪(CyTOF)在单细胞水平上建立ICC患者在化疗前、化疗中和化疗后指定时间点外周血单核细胞(PBMC)的免疫谱;应用多重免疫荧光染色展现某些特定免疫亚群的空间分布;使用组织微阵列(TMA)评估患者预后。本研究共招募了20名接受吉西他滨(GEM)治疗的ICC患者,其中8例对化疗反应良好(R),12例无反应(NR)。我们观察到化疗后患者PBMC组成发生了巨大变化,比如CD4/CD8 双阳性T细胞(DPT)水平增加。治疗前CD4+CD45RO+CXCR3+T细胞水平较高的患者对化疗反应良好。我们的研究确定了T细胞亚群的百分比与患者化疗反应性之间存在正相关关系,这表明通过评估PBMC中细胞亚群的比例来预测ICC患者化疗反应性是可行的。
标题 作者 时间 类型 操作
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
期刊论文
Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission
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期刊论文